APPLICATION NOTE
6
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0
10
20
ΔFnorm
[‰]
0.92
0.96
1.00
-1 0 1 2 3 4 5
Time [s]
Relative
Fluorescence
[-]
935
940
945
950
955
960
1x10
1
1x10
2
1x10
3
1x10
4
1x10
5
1x10
6
Fnorm
[‰]
Ligand Concentration
0.90
0.92
0.94
0.96
0.98
1.00
Relative
Fluorescence
[-]
-1 0 1 2 3 4 5
Time [s]
Single-dose screen
(A) ΔFnorm
(B) TRIC trace
Affinity screen
(C) Fnorm dose response curve
(D) TRIC trace
Fragment
Positive control SAM
(A) (C)
(B) (D)
-4
-2
0
2
4
6
ΔFnorm
0.92
0.96
1.00
Relative
Fluorescence
[-]
-1 0 1 2 3 4 5
Time [s]
932
936
940
944
Fnorm
[‰]
1x10
2
1x10
3
1x10
4
1x10
5
1x10
6
1x10
7
Ligand Concentration
0.90
0.92
0.94
0.96
0.98
1.00
Relative
Fluorescence
[-]
-1 0 1 2 3 4 5
Time [s]
Fragment A12935
(A) (C)
(B) (D)
