a
Here are the most common biophysical technologies and their strengths and weaknesses.
Surface Plasmon
Resonance
(SPR)
Isothermal
Titration
Calorimetry
(ITC)
BioLayer
Interferometry
(BLI)
MicroScale
Thermophoresis
(MST)
Requires high amount of sample No Yes No No
Label-free Yes Yes Yes Optional
Immobilization-free No Yes No Yes
High throughput Yes No Yes Yes
Easy to use No No Yes Yes
Instrument costs High Low Medium Medium
Consumable costs High Low Medium Medium
Measures binding kinetics Yes No Yes No
nanotempertech.com
Measure binding affinity with these technologies
