Technical Notes

Precision of nanoDSF measurements with Prometheus

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TECHNICAL NOTE Prometheus is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2019 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-PR-001-02 nanotempertech.com measured values, which means it allows to compare different types of systems that may have completely different output parameters. The RSD is also known as coefficient of variation (CV). The obtained values show the outstanding repeatability and intermediate precision routinely achievable with any instrument in the Prometheus platform. For more info visit nanotempertech.com/prometheus Figure 1: Overlay of 48 sample measurement from a single experiment. Table 1 gives an overview of the precision for the initial ratio and the T m calculated for 10 individual instruments as well as the average variation for the 48 sample positions across the 10 instruments. The table lists standard deviations (SD) and relative standard deviations (RSD). The RSD is useful as a dimensionless parameter that is independent of the magnitude of the

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