Dynamic Light Scattering (DLS)
Of all the Prometheus instruments, Prometheus Panta is the one
that uses DLS to detect molecule aggregates and determine the
size of proteins over a wide concentration range.
Use it to
• Screen recombinant proteins expressed from
different constructs
• Screen for biologics formulations
• Optimize samples for biophysical assays
• Characterize self-interactions
10
-9
10
-8
10
-7
10
-6
0.00
0.05
0.10
0.15
r
H
[m]
Relative
frequency
BGG in Histidine buffer
NIST mAb in Saline buffer
NIST mAb
BGG
Sample
5.5 ± 0.02
6.9 ± 0.3
r
H
PDI
0.07
0.2
Compare size distribution, mean r
H
, and PDI to identify optimal
buffer conditions for your sample and assess sample homogeneity
before performing other time-consuming biophysical assays.
