Backreflection
Prometheus uses backreflection to determine
aggregation or colloidal stability by measuring loss of
light intensity due to scattering.
Because thermal unfolding or conformational stability
doesn't always correlate with colloidal stability, use
backreflection simultaneously with nanoDSF for the
most precise characterization.
0.8
0.8 4
0.8 8
0.9 2
0.9 6
1
40 50 60 70 80 90
pH 4
pH 4.5
pH 5
pH 5.5
pH 6
pH 6.5
F350/F330
Temperature (°C)
100
200
300
400
500
600
40 50 60 70 80 90
pH 4.5
pH 5
pH 5.5
pH 6
pH 6.5
pH 4
Scattering
(mAU)
Temperature (°C)
See how thermal unfolding and aggregation are affected
by buffer formulations. Use the data to select the one that
ensures the best environment for your biologic.
Thermal unfolding with nanoDSF
Aggregation with backreflection
