The involvement of the immune system in tumor control is becoming well understood. The tumor environment is in a state of equilibrium between the elimination of cancer cells by the immune system and their proliferation. The immuno-evasion mechanisms that allow tumor cells to remain in equilibrium with the host depends on the overexpression of immunoregulatory molecules such as PD-L1 and its interaction with PD-1 expressed on tumor-infiltrating T lymphocytes. Blocking this interaction is being considered as a more targeted therapeutic approach that blocks the interaction of PD-1 with its ligands, leading to various clinical trial using monoclonal antibodies anti-PD-1 or anti-PD-L1 in several types of cancers. In order to develop such therapies, it is imperative to characterize the interaction between PD-1 and PD-L1. In this webinar we will show how we employed MicroScale Thermophoresis (MST) for the quantitative analysis of the binding affinity between these two molecules. Particularly we describe a method that utilizes low sample consumption and no tedious purification step of the protein of interest.
The stability of enzymatic biocatalysts is of primary importance in the biotechnology, food, chemical, and ...
Most recent content
Are you struggling to tame your mischievous membrane proteins? Find out how NanoTemper can help you to characterize even the most challenging membrane protein samples. You will learn how Prometheus ca
Monoclonal antibodies and therapeutic proteins for vaccines require extensive stability characterization during their development phase to improve their probability of successful treatment. We will di
Bifunctional degrader molecules (also known as PROTACs) and molecular glues recruit proteins to E3 ubiquitin ligases. The formation of a ternary complex between PROTAC, ligase, and the protein of inte
The stability of biopharmaceuticals is a complex mixture of parameters and plays a crucial role throughout the entire product lifecycle, from discovery through production and manufacturing. Making cri
Stability of biopharmaceuticals is a complex matrix of parameters that plays a crucial role through the entire product life-cycle. Through case studies, we discuss how Prometheus provides clarity by d
The prominent role of glycans in biomedical research is exponentially raising as researchers discover how these sugars deeply affect protein structure and function.
Development of therapeutic antibodies is a long and costly process. One key indicator of whether biologic candidates will be successful is stability. Prometheus enables rapid and precise characterizat
New advances in gene therapy have triggered a resurgence of interest in the field. Though gene therapy offers exciting potential for the treatment of both monogenic disorders as well as more genetical
There are a number of interesting drug targets that have a reputation of being especially difficult to study in biochemical or biophysical assays. Join us to learn how Dianthus can help you address t
As the population grows, our environment is changing and our natural resources are being depleted. That’s why research on plant production, temperature and pathogen resistance, environmental stress, p
Neurodegenerative diseases like Alzheimer’s or Parkinson’s Disease affect millions of people worldwide. Although current treatments may help relieve some of the physical or mental symptoms, there is s
Pandemics have affected human society throughout history, but with the current pandemic happening today, realization is quickly setting in just how much viral infectious diseases impact everyday life.
Gene therapy—a type of DNA-based medicine that utilizes a virus to insert a healthy gene into cells to replace a mutated, disease-causing variant has emerged as one of the most promising medical treat
How do you usually know whether your protein is happy? Even if you’re already using a portfolio of techniques to check, you may not have all the info. Find out how to use Tycho to really understand wh
This therapeutic antibody drug development webinar discusses the idea of limiting the substantial investment of time, money, or resources.
A big hurdle in small molecule drug discovery screening is confidently identifying positive hits in a timely manner. Screening fragment libraries is challenging with current methodologies that are eit
Vaccines are biological products derived from living organisms that provide an immune response to pathogens or diseases.While research efforts in developing vaccines continue to increase, scientists
Cellular expression and purification of proteins are among the most widely used techniques in biology. Expressing proteins in stable, properly-folded states is a challenge that may be abated by monito
Process development for Fill & Finish activities of large molecule parenterals requires an orchestrated effort of multiple functions to deal with product specific critical quality attributes (CQAs) an