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Characterizing binding interaction between PD-1/PD-L1 directly from cell lysates using MST

The involvement of the immune system in tumor control is becoming well understood. The tumor environment is in a state of equilibrium between the elimination of cancer cells by the immune system and their proliferation. The immuno-evasion mechanisms that allow tumor cells to remain in equilibrium with the host depends on the overexpression of immunoregulatory molecules such as PD-L1 and its interaction with PD-1 expressed on tumor-infiltrating T lymphocytes. Blocking this interaction is being considered as a more targeted therapeutic approach that blocks the interaction of PD-1 with its ligands, leading to various clinical trial using monoclonal antibodies anti-PD-1 or anti-PD-L1 in several types of cancers. In order to develop such therapies, it is imperative to characterize the interaction between PD-1 and PD-L1. In this webinar we will show how we employed MicroScale Thermophoresis (MST) for the quantitative analysis of the binding affinity between these two molecules. Particularly we describe a method that utilizes low sample consumption and no tedious purification step of the protein of interest.
Previous Video
Identifying novel biocatalyst candidates via high-throughput stability monitoring
Identifying novel biocatalyst candidates via high-throughput stability monitoring

The stability of enzymatic biocatalysts is of primary importance in the biotechnology, food, chemical, and ...

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Understanding the role of assembly factors in ribosome biogensis
Understanding the role of assembly factors in ribosome biogensis

Ribosomes are massive macromolecular complexes composed of proteins and RNAs andperform one of the most fun...

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