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Due to the high precision and resolution, nanoDSF poses an attractive alternative to differential scanning calorimetry experiments, which also deliver high resolution unfolding data but require a significantly larger amount of protein and time for sample preparation, as well as less throughput and a more limited concentration range.
The nanoDSF method employed by the Prometheus delivers highest quality unfolding data for antibody buffer screening campaigns. The high-resolution of the thermal unfolding curves is instrumental for biophysical characterization of antibodies since the effects of buffers, excipients or even covalent modifications can easily be assigned to distinct antibody domains.