Accelerate your protein purification workflow by knowing protein quality first

January 23, 2020 NanoTemper Technologies

Protein quality is a challenging parameter to measure. Clearly, when the quality of a protein is poor, it likely won’t function and often can be difficult to even characterize. When questionable or non-reproducible results arise, the quality of a protein should be questioned. For most researchers, the typical routine is to repeat experiments again and again, while assuming that the sample they’re using — whether prepared by themselves, a colleague or commercially purchased — is completely fine.

For decades, protein quality has been assessed by traditional tools, such as SDS-PAGE and UV/VIS absorbance. However, these traditional tools are time-consuming and often have to be used in tandem to provide more comprehensive results on your sample.

With Tycho, you can get multiple results on the quality of your protein sample — including presence, purity, similarity, functionality, and concentration — all within one single experiment. Measurements can be done as quickly as 3 minutes, in a label-free way, using just 10 microliters of sample.

In this on-demand webinar, Tom Cottle from the Harvard/Beth Israel Deaconess Medical Center X-Ray Crystallography Core explains how Tycho has been an asset in helping him improve his purification workflows by:

  1. Rapid protein identification throughout a purification workflow
  2. Optimization of purification conditions, including refolding, buffers, and storage by monitoring protein quality
  3. Confirmation of protein complex formation and small molecule effects on protein stability

To learn more, watch the on-demand webinar and find out how Tycho can be your everyday solution to monitor protein quality

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