The result is broader interaction data across challenging targets, even when sample is limited.

Monolith Omni is a benchtop biophysical platform from NanoTemper Technologies that combines affinity, kinetics, and qualitative stability analysis in a single in-solution workflow. It is a new addition to the Monolith platform, designed to help researchers build a more complete picture of biomolecular interactions - from the same sample, without additional material.

Monolith Omni was built to help answer them in one workflow with minimal sample consumption.

 When targets are challenging and sample is limited, a binding affinity value is a starting point, not a conclusion. K_d can tell you whether two molecules interact, but it cannot tell you how quickly binding happens, how long a complex remains intact, or whether your target stays stable under assay conditions. Those questions matter in drug discovery, protein biochemistry, and molecular interaction research.

Multi-parameter interaction characterization in a single in-solution workflow.

What is Monolith Omni?

Monolith Omni: Affinity, Kinetics, and Stability.
One Workflow, One Sample.

 Two compounds can show similar affinity and still behave very differently in practice. One may bind quickly and dissociate just as fast. Another may show longer residence time or stabilize the target in a way the first does not. In academia, this can help researchers better understand mechanism and binding behavior. In biopharma, it can support hit validation, lead optimization, and candidate selection.

Monolith Omni brings these layers of information together in one in-solution workflow. 

• Kinetic rates (k_on, k_off) reveal whether a drug candidate binds fast enough and stays bound long enough to be therapeutically relevant.

• Stability data shows whether your target is folded and functional, and whether a ligand stabilizes or destabilizes it.  

• Orthogonal confirmation increases confidence in hits and candidate decisions.

Affinity (Kd) tells you the strength of a binding interaction, but it doesn't explain how quickly a molecule binds, how long it remains bound, or how stable your target is under assay conditions. These parameters, kinetics and stability, are often decisive in drug discovery and molecular research.

Why isn't affinity measurement alone enough?

 A defining capability of Monolith Omni is in-solution kinetic characterization using nanoTAK (nano Temperature Alteration Kinetics). Unlike traditional surface-based methods such as SPR or BLI, Monolith Omni measures interactions in solution, without immobilizing either binding partner. This helps reduce surface-related effects and mass-transport limitations that can complicate interpretation in surface-based assays. 

nanoTAK takes a first-principles and well characterized and accepted approach to kinetics measurement by perturbing a pre-equilibrated sample and monitoring its shift to a new equilibrium position in real time. A rapid and precise shift in sample temperature is created using an IR laser, perturbing the equilibrium, and the resultant changes in Spectral Shift report the resulting relaxation behavior, from which kinetic rate constants can be extracted, fully in solution.

How does Monolith Omni measure kinetics in solution?

• Membrane proteins - in detergent, nanodiscs, or complex unpurified matrices

• Intrinsically disordered proteins (IDPs) and transcription factors

• Nucleic acids - DNA, RNA, aptamers, antisense oligonucleotides

• Small molecules and fragments, including weak binders in the mM range

• Molecular glues, PROTACs, and degraders - including ternary complex cooperativity and hook effect analysis

• Covalent inhibitors - measured at equilibrium via direct biophysical readout

Monolith Omni is designed for challenging targets and diverse molecular modalities that are difficult to measure with surface-based methods. Supported targets include:

What types of targets can Monolith Omni characterize?

This low sample requirement is especially valuable for difficult-to-express proteins, precious purified material, membrane proteins, and early-stage candidate molecules available only in limited quantities.

• 10 µL of sample per capillary

• Up to 24 capillaries per run

• Multiple readouts from the same sample

When material is scarce, every microliter matters. Monolith Omni is designed to help researchers When material is scarce, every microliter matters. Monolith Omni is designed to help researchers generate more insight from less sample.

Why is Monolith Omni well suited for sample-limited experiments?

Monolith Omni is designed for straightforward onboarding. Installation and user training can be completed in a single day, and the workflow is supported by guided software for assay setup, optimization, and real-time data acquisition. Single-use capillaries help avoid cross-contamination and reduce maintenance, while the fluidics-free design keeps the workflow simple.

How quickly can labs bring Monolith Omni into their workflow?

• Your target is challenging: membrane protein, IDP, nucleic acid, or a modality that struggles with surface immobilization.

• Sample is limited and needs to go as far as possible.

• You're working with molecular glues, PROTACs, or degraders where ternary complex kinetics matter.

• You need orthogonal hit confirmation without running a separate experiment. 

• You want kinetic context to support lead optimization decisions.

Monolith Omni is designed for researchers in academia and biopharma who need more than affinity data to make confident decisions about their candidates. It is particularly well suited when:

Who is Monolith Omni for?

What does Monolith Omni measure?
Monolith Omni measures binding affinity (K_d), kinetic rate constants (k_on/k_off​), thermodynamic parameters via Van’t Hoff analysis, and qualitative protein stability in solution within a single workflow.

How is Monolith Omni different from other kinetics platforms?
Unlike surface-based kinetics platforms such as SPR or BLI, Monolith Omni measures interactions in solution without immobilizing either binding partner. This helps reduce surface-related artifacts, supports challenging targets, and enables kinetic analysis in a fluidics-free workflow. 

How much sample does Monolith Omni require?
Each measurement requires 10 µL of sample per capillary, with up to 24 capillaries per run.

Can Monolith Omni measure membrane proteins?
Yes. Monolith Omni supports membrane protein characterization in detergent, nanodiscs, or complex unpurified matrices, which can be challenging for some surface-based methods.

Can Monolith Omni measure molecular glues and PROTACs?
Yes. Monolith Omni supports analysis of heterobifunctional degraders, including binary and ternary complex affinities, cooperativity, and hook effects. Is

Monolith Omni suitable for high-throughput screening?
Monolith Omni is designed for deep, multi-parameter characterization rather than large-scale screening. It is best suited for applications where interaction quality, limited sample, and workflow simplicity are priorities.

Where can I learn more or request a demo?
Visit the Monolith Omni page to explore the platform, download the data file, or speak with a specialist.

 FAQs

It supports workflows from early hit validation through lead optimization, as well as broader molecular interaction studies in academic research. 

"Monolith Omni isn't just a product launch, it opens a new chapter for NanoTemper. It helps scientists move beyond data collection toward a deeper understanding of molecular interactions, with less friction, less sample, and a faster time to insight. For teams working on tough questions, that's a meaningful step forward." — Dr. Stefan Duhr, CEO, NanoTemper Technologies