1 2
A P P L I C A T I O N N O T E
Hit Picking and Prioritization. Each plate was normalized using the Robust Z score method [14],
using the DMSO negative control as reference, and single point data visualized as in Figure 4. As
primary screening was performed in duplicate, hits were classified as described in Figure 4. Tier 1
through 3 hits (Prio 1) were hits where both repeats had a consistent and reproducible signal (Figure
5A). An additional 81 compounds (Tier 4/Prio 2) showed activity in one repeat alone, which were also
taken forward for dose response testing.
Figure 4. n=2 Single Point Screen Summar y Plot. Each plate was normalized to DMSO using the robust Z score
method, and absolute robust Z score values plotted. Hits prioritized as shown on right hand side of figure. 4 RSDE
indicated in pink dashed line, 5 RSDE indicated by yellow dashed line.
