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A P P L I C A T I O N N O T E
Figure 8. ATPase inhibition screening of Prio 1 and Prio 2 compounds. A. Histogram showing the DMSO reference
(blue), U0126 Reference (orange) and Prio 1 compounds. B. Affinity of Prio 1 compound Spectral Shift Robust Z Score
plotted against ATPase Activity Inhibition. C. Prio 1 weak and strong binders (where K
d
< 35 µM) plotted against ATPase
Activity Inhibition. An inhibition cut off of 3 σ of DMSO negative control (7.66 %), grey dashed line was used to classify if
inhibition of ATPase activity was statically significant.
