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A P P L I C A T I O N N O T E
MUST-HAVE FEATURES FOR TERNARY COMPLEX ASSAYS
Hans-Jörg also outlines the key features he looks for when evaluating technologies for ternary
complex assays. These include:
• Sensitivity: The technology must be sensitive enough to detect weak affinities with a high signal-
to-noise ratio, ensuring accurate readings even at low protein concentrations.
• In -Solution assays: Avoiding the immobilization of proteins minimizes artifacts and allows for
high compound concentrations. Solution-based assays provide more accurate and consistent
results when working in complex conditions.
• Throughput: While screening millions of compounds may not be necessary, medium to high
throughput is essential for processing hundreds or thousands of hits efficiently, particularly in
hit characterization.
• Minimal Protein Labeling: Technologies that don't require labeling of all involved proteins are
preferable, as they are less prone to artifacts and therefore provide more reliable results, being
closer to the native system.
Highlights
U S E R C A S E S T U D Y
