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A P P L I C A T I O N N O T E
Figure 2. Dianthus with Spectral Shift technology.
DIANTHUS: ADVANCING COOPERATIVITY SCREENING
To address the challenges in proximity inducer screening, Dianthus integrates cutting-edge Spectral
Shi technology, which was specifically designed to overcome the limitations of traditional binding
assays. According to Hans-Jörg, the selection of this method for some difficult projects, came out
of a need to monitor weak target binding. "We tried the usual methods like SPR and FRET, but they
failed to measure K
d
s greater than 50 μM.", Hans-Jörg shared. It was this persistent issue that led him
and his team to turn to Spectral Shi technology.
The key advantage of this method, Hans-Jörg notes, is that it is a pure target engagement technique.
Unlike other affinity assays that require tagging both proteins — such as FRET — Spectral Shi
technology monitors only the target protein. "It's a direct measurement of changes on the target
itself, without the need for labeling both proteins, making the assay easier and more precise," Hans-
Jörg explains. This results in a more accurate and sensitive way to characterize proximity inducers,
particularly when assessing the cooperativity of these ternary complexes.
U S E R C A S E S T U D Y
