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A P P L I C A T I O N N O T E
Ensuring that membrane proteins retain their native conformation throughout biophysical analysis
is paramount to study their structure-function interactions. The Monolith X instrument measures
mass-independent biomolecular interactions in solution, with any buffer composition including ex-
cipients, and using minimal sample; these attributes are idyllic for a variety of membrane protein-re-
lated interactions, including protein-small molecule, protein-peptide, and protein-protein interac-
tions that have proven difficult using other technologies.
In the following sections, this application note will underscore specific examples that illustrate the
implementation of combining NativeMP™ Technology and the Monolith X instrument for membrane
protein interaction studies, highlighting the effectiveness and versatility of this application for ad-
vancing biophysical characterizations and accelerating new therapeutic discoveries.
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Figure 2: Monolith X with spectral Shift technology for binding affinity studies.
