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A P P L I C A T I O N N O T E
Quantifying binding affinities is fundamental for understanding how structure and function influ-
ence biomolecular interactions; NanoTemper Technologies' Monolith X instrument, with its dual
measurement modes Spectral Shi¤ and TRIC, defines a new standard capable of handling delicate
or low-abundance membrane proteins with precision, reliability, and ease-of-use.
Pairing the Monolith X instrument with Cube Biotech's NativeMP™ Technology offers a unified work-
flow that minimizes sample preparation while preserving the structural and functional integrity of
membrane proteins. These innovative technologies enable a highly effective investigation of diverse
binding interactions, such as protein-small molecule, protein-peptide, and protein-protein affini-
ties, affirming this combinatorial approach as an indispensable tool for researchers in drug discov-
ery, development, and biophysical analysis.
Conclusion
