1
A U T H O R S
Philipp T. Hanisch
1
, Lea Marie Esser
1
, Sergej Balanda
1
,
Nishika Sabharwal
1
, Anny Fis
2
, Ping Zhang
2
1
Cube Biotech GmbH, Monheim-am-Rhein, Germany
2
NanoTemper Technologies GmbH, Munich, Germany
A B S T R A C T
Membrane proteins are critical in drug discovery due to their central role in cellular communication and
signaling, making them key therapeutic targets for next generation medicines. However, their unique
properties pose significant challenges for biophysical characterization, particularly in maintaining stabil-
ity and native conformation outside of biological membranes. The stringent buffer conditions required
to maintain the integrity of membrane proteins when investigating their structure and function are lim-
iting, highlighting the need for innovative technologies that don't rely on traditional detergent-based
approaches to facilitate novel drug discovery and drug interaction analysis with membrane proteins.
This application note underscores the combined strength of using the latest technologies from
Cube Biotech and NanoTemper Technologies to supersede these challenges. NativeMP™ Technology,
developed by Cube Biotech, utilizes detergent-free synthetic copolymers to stabilize membrane pro-
teins in their native environment, while the Monolith X instrument from NanoTemper Technologies em-
ploys Spectral Shift and TRIC technologies to directly measure biomolecular interactions in solution;
coupling these technologies empowers researchers to obtain highly sensitive and precise binding affin-
ities while preserving the protein's native conformation during measurements.
A P P L I C A T I O N N O T E
A C H I E V E H I G H LY S E N S I T I V E A N D P R E C I S E B I N D I N G A F F I N I T Y M E A S U R E M E N T S
W I T H O U T D I S R U P T I N G P R O T E I N C O N F O R M AT I O N
Redefine Membrane Protein Research
Utilizing NativeMP™ Technology and
the Monolith X
K E Y W O R D S
Membrane proteins
Synthetic nanodiscs
Copolymer
Spectral Shift
Binding affinity
Native conditions
NativeMP
TM
