Issue link: https://resources.nanotempertech.com/i/1526380
1 A B S T R A C T Cryogenic Electron Microscopy (cryo-EM) has enabled atomic-level structure determination of many complex proteins that were not previously achievable with X-ray crystallography or nuclear magnetic resonance (NMR). Membrane proteins, while being critical targets for drug development, present additional challenges for structural analysis; their amphipathic nature, complex architecture, as well as tedious expression and purification continue to strain researchers working with these proteins. With the use of innovative solubilization approaches, such as synthetic copolymers and the NativeMP TM Platform, companies like Cube Biotech can more efficiently create soluble membrane proteins optimized for cryo-EM workflows to support structural biologists. In this work, we describe how the application of dynamic light scattering (DLS) and nano Differential Scanning Fluorimetry (nanoDSF) provided by the Prometheus Panta helps membrane protein structural biologists rapidly find the best copolymers for their synthetic nanodiscs to ensure the highest atomic resolution for cryo-EM structures. A P P L I C A T I O N N O T E O P T I M I Z AT I O N O F M E M B R A N E P R O T E I N S F O R C R Y O - E M Screening of copolymer nanodiscs with multi-parameter stability characterization on the Prometheus Panta K E Y W O R D S Membrane proteins, cryo-EM, synthetic nanodiscs, copolymer, DLS, nanoDSF, Contract Research Organisation (CRO), NativeMP TM A U T H O R S Philipp T. Hanisch 1 , Michael Ries 1 , Greg Dodge 2 , Léa Valla 3 , Ping Zhang 3 1 Cube Biotech GmbH, Monheim-am-Rhein, Germany 2 Massachussets institute of Technology (MIT), Imperiali Group, Boston, United States 3 NanoTemper Technologies GmbH, Munich, Germany