However, no shi in T
m2
(Fab/Fc2) and a
very similar profile otherwise indicates
that the conjugation process le the mAb
otherwise intact
The destabilizing shi in T
m1
indicates
that the small molecule drug is conjugated
to the Fc1 domain
F350/F330
.95
0.9
0.85
0.8
0.7
0.75
30 40 50 60 70 80 90
ADC
naked mAb
Temperature (°C)
0.025
0.015
0.010
0.005
-0.005
0.000
30 40 50 60 70 80 90
ADC
naked mAb
Temperature (°C)
0.020
100 20
The conjugation process o en
introduces harsh or destabilizing
conditions to an antibody that will
have a negative impact on its long-
term stability and developability.
By comparing the thermal
unfolding profiles of the final ADC
candidate with the orginal, naked
(unconjugated) mAb, you learn
whether your therapeutic remained
stable during the process, and even
confirm the domain to which the
drug conjugated.
100
First
derivative
