High-resolution data enables precise modelling to show exactly how much
a biosimilar deviates in thermal stability from its originator. With this info, it's
possible to find a candidate that is highly similar to the original molecule.
A mathematical model finds
overlap of the first derivative
plots of the biosimilar samples
against the originator for fast
ranking of the best candidates
Sample
1st Derivative
Overlap Index
Rank
1
0.492 17
2 0.461 13
3 0.306 2
4 0.446 9
5 0.488 16
6a 0.461 14
6b 0.423 6
7 0.458 12
8 0.403 4
9 0.433 7
10 0.438 8
11
0.111 1
12 0.464 15
13 0.455 11
14 0.452 10
15 0.332 3
16 0.423 5
50 60 70 80 90
36BLX
35BLX
33BLX
34BLX
37BLX
90
36BLX
35BLX
33BLX
34BLX
37BLX
Sample 1 - worst match Sample 11 - best match
70 80 90
[°C]
36BLX
35BLX
33BLX
34BLX
37BLX
First
Derivative
0.82
0.84
0.86
0.88
0.9
0.92
0.94
0.96
0.98
1
Ratio
350
nm
/
330
nm
40 45 50 55 60 65 70 75 80 85
Temperature [°C]
Sample1
Ref(1)
11
First
Derivative
0.82
0.84
0.86
0.88
0.9
0.92
0.94
0.96
0.98
1
Ratio
350
nm
/
330
nm
40 45 50 55 60 65 70 75 80 85
Temperature [°C]
Sample1
Ref(1)
11
First
Derivative
0.82
0.84
0.86
0.88
0.9
0.92
0.94
0.96
0.98
1
Ratio
350
nm
/
330
nm
40 45 50 55 60 65 70 75 80 85
Temperature [°C]
Sample1
Ref(1)
11
First
Derivative
0.82
0.84
0.86
0.88
0.9
0.92
0.94
0.96
0.98
1
Ratio
350
nm
/
330
nm
40 45 50 55 60 65 70 75 80 85
Temperature [°C]
Sample1
Ref(1)
11
Originator molecule
Read more about the modelling for determining first derivative overlap in this app note
