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The only way to get binding affinities in solution using very minimal sample

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For one reason or another, SPR has a difficult time analyzing challenging binding events no matter how many times you've tried to develop an assay. When you find yourself in these situations, turn to Monolith X to help. Work with challenging interactions that are difficult for SPR Challenge It's impossible to regenerate biosensors when working with covalent analytes Solution Each measurement is done in solution so it's easy to measure covalent interactions. There's no need to figure out how to regenerate the surface like with SPR. Challenge You experience non-specific binding between the analyte and matrix Solution You'll never have to worry about or test for non- specific binding since measurements are done in solution. Challenge Immobilization prevents you from getting a K d Solution With measurements in solution, you'll get a K d for IDPs, membrane proteins, and other challenging molecules.

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