For one reason or another, SPR has a difficult time analyzing challenging binding events
no matter how many times you've tried to develop an assay. When you find yourself in
these situations, turn to Monolith X to help.
Work with challenging interactions that are difficult for SPR
Challenge
It's impossible to regenerate
biosensors when working
with covalent analytes
Solution
Each measurement is done in
solution so it's easy to measure
covalent interactions. There's
no need to figure out how to
regenerate the surface like with
SPR.
Challenge
You experience non-specific
binding between the analyte
and matrix
Solution
You'll never have to worry
about or test for non-
specific binding since
measurements are done in
solution.
Challenge
Immobilization prevents you
from getting a K
d
Solution
With measurements in
solution, you'll get a K
d
for
IDPs, membrane proteins,
and other challenging
molecules.
