Get the information you need to
profile your gene therapy vectors
Prometheus Panta utilizes 4 different technologies —
nanoDSF to create thermal profiles of your vectors, DLS
to assess their purity and assembly, SLS for determining
genetic fill, and backreflection for monitoring
aggregation. Each one tells a different piece of the story
about your vector's behavior. But they have one thing in
common — they're all label-free.
Serotyping
Thermal profiling with nanoDSF
allows you to confirm batch-to-batch
similarity in your AAV production
scheme. Also, see how production
changes affect the overall thermal
stability of your preparations.
Vector stability
enhancement
DLS shows you how clean your
preparations are, so that you are
confident you're working with
high-quality product. Insufficient
clean-up processes can lead to
unwanted byproducts that lead to
lowered efficacy of your gene therapy
products.
Capsid assembly and
packaging
SLS determines the molecular weight
of your particles to ensure they
have assembled correctly. Proper
assembly of your AAVs is necessary
for packaging your gene therapy
products.
Genetic fill
Both nanoDSF and SLS are tools
to monitor the amount of genetic
material a vector carries. Creating the
perfect therapeutic vector requires
you to not only optimize its capsid,
but also determine if it has the
necessary genetic payload.
Storage and quality control
Prometheus Panta allows you to
compare your preps throughout every
step of the workflow, and ensure
you're working with a high-quality,
stable product. Storage conditions
have an impact on the efficacy of your
gene therapy vectors.
Formulation
Find which formulation conditions
provide the best stability for
your vectors with nanoDSF and
backreflection. Changes to the buffer
or stabilizing mutations in viral
particle proteins affect how long your
products last in cold storage and how
efficiently they deliver their genetic
payload.