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APPLICATION NOTE
demonstrating unfavorable biophysical characteristics. Other important biological
properties, such as the pI of the protein or its hydrophobicity index, have impact
downstream when it comes to packaging of the material or delivery of the therapeutic
to the patient. Classifying a library with extensive genetic variation is crucial for
understanding early discovery candidates and how to ultimately optimize them for long-
term success in the clinic.
For evaluation of some basic biophysical characteristics, Prometheus Panta can give
you important parameters such as T
on
,T
m
, T
agg
, and PDI, which can not only give a
biophysical picture of antibody libraries, but also predict the long-term stability of a given
antibody. Here we have demonstrated the value of these parameters for ranking
candidates, as well as that the nanoDSF and turbidity-derived data from the Panta
corroborates well with previously obtained data from the Prometheus NT.Plex. With
this information, we can conclude that the Panta instrumentation can be integrated into
workflows with groups that have already collected data on the NT.Plex.
