Application Notes

©2021 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. 7 APPLICATION NOTE Evaluating and comparing data from Promethus Panta vs NT.Plex One of the most important tenets of research is independent reproducibility. At large pharma companies such as Merck, stability parameters may be tested by different individuals across multiple sites, and it is crucial that these results can be independently verified. With the recent integration of DLS measurements in the Prometheus line, it was necessary to evaluate the reliability of the nanoDSF and turbidity data obtained from different systems. Previously published and evaluated data from Prometheus NT.Plex instrumentation must be replicated on the Panta to ensure it can be fully integrated into already established protocols. The mAbs in this study were previously evaluated using the Prometheus NT.Plex, with the same experimental parameters for nanoDSF and turbidity measurements, but by a different researcher. Figure 3 shows a few representative corroborations between the individual data points collected on the Panta vs. the NT.Plex. There is high correlation for the candidates evaluated, demonstrating good agreement between the new instrumentation and the established technology in the field. 55 60 65 70 75 80 85 PANTA T m1 (ᵒC) NT.Plex T m1 ( o C) 75 70 65 60 55 50 75 70 65 60 55 80 NT.Plex Tm 1 ( o C) PANTA T on (ᵒC) NT.Plex T on ( o C) 45 50 55 60 65 70 65 60 55 50 45 40 65 60 55 50 45 70 NT.Plex Tm onset ( o C) 30 40 50 60 70 80 90 100 PANTA T turb (ᵒC) NT.Plex T turb ( o C) 90 80 70 60 50 80 70 60 50 40 90 NT.Plex Tturb ( o C) 100 Figure 3: Figure 3 shows the correlations between onset of melting (A), first melting temperature inflection point (B), and onset of turbidity (C, particles >25 nm diameter) correlate well between the Panta and the NT.Plex. A B C

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