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4
APPLICATION NOTE
Figure 1: Profiles of parents from Group 1 (A) and Group 2 (B) antibody groups have unique thermal profiles from intrinsic nanoDSF (top), turbidity (center), and DLS
cumulant radius measurement (bottom).
Ratio
350
nm
/
330
nm
Turbidity
[mAU]
Cumulant
Radius
[nm]
Temperature [ºC]
A B
Ratio
350
nm
/
330
nm
Turbidity
[mAU]
Cumulant
Radius
[nm]
Temperature [ºC]
Protein A Protein B Protein C Protein D
Protein E
Protein F
Protein G
Protein H
Protein I
Protein J
T
m1
T
on
T
turb
T
agg
PDI (25ᵒC)
Protein A 64.99 60.48 64.33 63.42 0.1
Protein B 74.42 65.23 83.78 75.71 0.03
Protein C 70.13 63.47 78.48 79.16 0.01
Protein D 70.43 63.47 82.91 80.84 1.14
Protein E 63.91 54.65 59.95 60.41 0.33
Protein F 69.06 57.30 79.39 68.28 1.17
Protein G 71.47 65.26 82.97 82.34 0.03
Protein H 69.70 63.52 69.68 65.47 0.01
Protein I 69.69 62.61 76.77 70.71 0.04
Protein J 69.69 62.62 67.93 67.10 0.01
Table 2: Summary data of first unfolding
temperature, unfolding onset, turbidity
onset, scattering onset, and PDI for
each parent in two groups. A PDI <0.1
is considered highly monodisperse;
anything above 0.25 is considered
highly polydisperse -- high PDI values
highlighted in red.
