5
a. Structural biology
b. Multi-angle light scattering
c. Analytical ultracentrifugation
While DLS is a valuable tool, it's o en crucial
to consider alternatives, whether to have an
orthogonal method for measurements or for
cost or practicality reasons. Compare DLS to
other methods that can be used to obtain sizing
information about your biologics candidates.
5
Alternative Models
Once you've determined that DLS is the method for
you, it's time to set up a measurement. As with all
experiments in a lab, it's important to think about how
to best design an experiment, and how to treat your
samples before loading them up for analysis. Review
this section to learn some important considerations
and caveats that come with DLS measurements.
6
Practical considerations
a. Limitations
b. Experimental purpose
b. Sample prep
c. Absolute vs relative size
d. How formulation can affect measurements
8
Resources
7
Key Takeaways
Table of Contents
