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The biologics researcher's guide to DLS

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25 Intensity signal is observed at different time shi s, tau. Particles move differently based on their size, and will induce a different shaped ACF decay. From this decay function we can determine D, the diffusion coefficient, and therefore the particle size, r H (see Page 21). Time Time Time τ Time τ Correlation g 2 Correlation g 2 Correlation g 2 Correlation g 2 Intensity Intensity Large Particle Small Particle Time τ [log-scale] Time τ [log-scale] Autocorrelation Function DLS evaluates intensity fluctuations via the autocorrelation function 0 1 2 0 1 2

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