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The biologics researcher's guide to DLS

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15 There are two kinds of scattering relevant for DLS. Both of them are considered "elastic" scattering, which means there is no loss in the energy of the photon – i.e. the wavelength does not change when it encounters the particle in solution. The first is Rayleigh scattering. This is a phenomenon that occurs when a particle is much smaller than the incident light wavelength, typically less than 1/10th. In this scattering phenomenon, the radiated light scatters about evenly in all directions – i.e., the intensity does not significantly change based on its reflection angle. Note that there is a slight "node" in light scattering at angles 90 o to the incident light, hence it is not a perfectly even phenomenon. Scattering phenomena

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