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The biologics researcher's guide to DLS

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31 Air bubbles in the sample will give irregular autocorrelation data, resulting in fits with large error rates. Both ACF fits will struggle to fit, and there may be corresponding increases in the scattering intensity: Aggregation and sedimentation can affect the intensity of the sample. If large aggregate particles settle prior to measurement, there will be a large increase in the scattering intensity. The ACF will not reach baseline, as these large particles do not move in solution: 1 1.1 1.2 1.3 1.4 Amplitude 1.2 1.3 1.4 1.5 1.6 1.7 1 μs 100 μs 1 s 10 ms Tau Amplitude

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