30
The autocorrelation fit will be plotted using the ACF data available
(see
Page 24). If the sample is low quality (e.g., highly polydisperse,
has contaminants, or is not at high enough concentration for
measurement) this will be reflected by data points not lining up
well against the calculated fit.
Dirt and dust appear as particles on the intensity distribution, but show a poor cumulant radius fit
with the ACF. Replicates will not match up well because dirt and dust do not have regular sizes:
1
0
0.05
0.1
0.15
0.2
3.162 10 31.623
Radius [nm]
Intensity
distribution
[%]
100 316.228 1000 3162.278
Buffer size distribution
Uncontaminated
Contaminated
1.1
1.2
1
1.3
1.5
1.4
1.6
1.7
Amplitude
1 μs 100 μs 10 μs 10 ms 100 ms 1 ms
Autocorrelation Function
Tau
