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The biologics researcher's guide to DLS

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17 DLS typically uses incident light in the visible to near infrared spectrum. As the wavelength gets shorter, the light becomes more intense. As described in the previous section, DLS uses elastic scattering, so the wavelength and intensity do not change upon interacting with the particles in solution. Thus, bluer light will scatter more intensely for the same particle size. Wavelength of incident light in an instrument is a fixed parameter and does not change between experiments. Shorter (or "bluer") wavelengths give more scattering intensity. Wavelength

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