Choose Monolith to explore and optimize PROTACs
Get trustworthy results
Accurately measure both low binary and high
ternary binding affinities with Monolith's
broad dynamic range (pM – mM)
Eliminate uncertainty and ensure
you're working with a stable binary complex
Quantify interactions in-solution under
equilibrium to precisely control assay conditions
required to maintain your binary complex while
analyzing binding of its third component
Go further with the sample you have
Because Monolith is a highly sensitive
instrument, use only a low amount of
sample and use the rest for further
efficacy evaluations
Save time thinking through
your entire assay setup
Easily set-up your binary and ternary
interactions with step-by-step instructions
using Monolith's integrated so ware solution
for ternary complex analysis
nanotempertech.com/monolith
Accurate quantitative analysis of binary and ternary
binding affinities and rapid cooperativity assessment of
MZ1 with Brd2
BD2
and VCB using MST.
Ternary K
d
determination for MZ1-Brd2
BD2
-VCB (dark blue)
and binary K
d
determination of MZ1 and VCB (light blue).
α represents cooperativity.
Struggling with
designing an effective
protein degrader?
Having a method in place
that effectively evaluates
ternary interactions and
cooperativity is essential for
understanding the
structure–activity
relationship of PROTACs.
Use Monolith to help you
facilitate the rational design
of your PROTACs.
nanotempertech.com/monolith
Monolith and NanoTemper are registered trademarks of NanoTemper Technologies GmbH, Munich, Germany.
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