Details for the
techie in your lab
nanoDSF
Measurement parameters
Ratio: T
onset
, T
m
, ΔG, ΔΔG, E
a
, reversibility of unfolding
330 nm: T
m
350 nm: T
m
Concentration range 5 μg/mL - 250 mg/mL
Inflection point precision @ 75 °C ± 0.1 °C
Ratio precision/reproducibility 0.008
DLS
Measurement parameters
T
scattering
, T
size
, r
H
, PDI, k
D
, D
0
, average scattering intensity,
reversibility of unfolding
Laser wavelength 405 nm ± 5 nm
Concentration range 0.5 mg/mL for a 15 kDa protein, up to 40% w/v
Size resolution Down to 0.5 nm
Backreflection
Measurement parameters T
turbidity
, reversibility of unfolding
Size resolution Larger than 12.5 nm radius
Technology Specifications
