Monolith uses MST technology to quantify molecular
interactions between a target and ligand by detecting changes
in fluorescence intensity while a temperature gradient is
applied over time (grey box, top figure). The fluorescent signal
comes from the target that is either fluorescently labeled
or has intrinsic fluorescence and becomes an extremely
sensitive reporter for the interaction. The binding affinity
is automatically determined at the end of each run without
additional and lengthy data analysis. The affinity constant
(K
d
) is calculated from a fitted curve that plots normalized
fluorescence against concentration of ligand (bottom figure).
Use MST technology to
measure interactions
-5 5 10 15 20
Time (seconds)
750
800
850
900
950
1000
F
norm
(‰)
0
1 10 100 1000
Ligand (nM)
810
820
830
840
850
F
norm
(‰)
K
d
