Measure high and low
affinities in the same system
Different projects have different
demands. It's inevitable that you'll
need to look at both weak and strong
molecular interactions. With a broad
sensitivity range, Monolith measures
K
d
s from pM to mM, so you can tackle
as many projects as you want.
Measure in solution, in
close-to-native conditions
Immobilizing molecules in SPR assays
o en causes them to lose activity, and
you're le without results. Because
Monolith measures in solution in most
buffers, both binding partners are free
to interact in their native conformation,
you'll finally get results.
Characterize binding events
with very little sample
It's not easy to prepare the large
volumes of highly concentrated target
and ligand required by ITC. With
Monolith, you get a K
d
in a fraction of
the volume and concentration, leaving
you with more sample for additional
experiments.
Monolith measures the broadest range
of binding affinities in-solution, using
very little sample
