Technical Notes

Accelerate your assay development using the enhanced Binding Check module in Monolith's MO.Control 2 software

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Monolith and NanoTemper are registered trademarks of NanoTemper Technologies GmbH, Munich, Germany. ©2020 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-MO-003-01 nanotempertech.com References 1 Bartoschik T, Galinec S, Kleusch C, et al. Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis. Sci Rep. 2018;8(1):4977. doi:10.1038/s41598-018-23154-3 2 Jerabek-Willemsen M, André T, Wanner R, et al. MicroScale Thermophoresis: Interaction analysis and beyond. J Mol Struct. 2014;1077:101-113. doi:10.1016/j.molstruc.2014.03.009 3 Khavrutskii L, Yeh J, Timofeeva O, et al. Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis. J Vis Exp. 2013;12(78):1-6. doi:10.3791/50541 4 Lippok S, Seidel SAI, Duhr S, et al. Direct Detection of Antibody Concentration and Affinity in Human Serum Using Microscale Thermophoresis. Anal Chem. 2012;84(8):3523-3530. doi:10.1021/ac202923j 5 Kemper Talley EA. On the pH-optimum of activity and stability of proteins. Proteins Struct Funct Bioinforma. 2010;78(12):2699-2706. doi:10.1038/jid.2014.371 6 Prodromou C, Roe SM, O'Brien R, Ladbury JE, Piper PW, Pearl LH. Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone. Cell. 1997;90(1):65-75. doi:10.1016/S0092-8674(00)80314-1

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