Monomeric vs.
oligomeric
Studying small molecule or
protein interactions in ND
requires working under
conditions that either favor
the monomeric or
oligomeric state of these
molecules.
Fast conformational
dynamics
Proteins involved in the onset
and progression of
neurodegenerative diseases
(ND) exist in different
conformational states - as
monomers or oligomers.
Their characterization is very
challenging because they can
transition from one state to
the other very fast.
High molecular weight
aggregates
Misfolded proteins escape
cellular quality controls and
form aggregates that have
the potential to compromise
cell function. Because they
can have high molecular
weight, these aggregates are
tricky to work with.
Intrinsically disordered
proteins
Intrinsically disordered
proteins (IDP) - those without
rigid 3D structures - are
associated with many ND.
Their structure changes
depending on the
environment or ligands,
which makes is very difficult
to work with them.
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With NanoTemper tools,
setup and run times take only
a few minutes - so you can
work with proteins with fast
conformational dynamics
with confidence.
Many biophysical methods take
a long time to set up and run -
by the time the measurement is
done, your protein may have
changed its conformation.
Researchers need biophysical
methods that allow them to
work at low sample
concentrations to study the
monomeric state, and also at
high concentrations to
investigate the oligomeric
state.
NanoTemper offers tools that
perform measurements with
sample concentrations from
mM all the way down to pM.
So you can evaluate the
monomeric and oligomeric
states.
Studying IDP with
NanoTemper technologies is
so easy. You skip
immobilization and measure
in solution and in close to
native conditions.
Characterizing IDP with
biophysical methods that
require immobilization to a
solid support and put
limitations to the buffer
conditions can disturb IDP
folding state.
Evaluating high molecular
weight aggregates with
NanoTemper technologies is
simple because
measurements are
independent of the size and
mass of the binding partners.
In order to understand the
modulation of these
aggregates by measuring
binding affinities,
researchers must find
technologies that allow them
to study high molecular
weight aggregates.
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4 ways to help you overcome the limitations
of current methods in neurodegenerative
disease research
nanotempertech.com/neurodegenerative-diseases
NanoTemper bioanalytical tools empower
neurodegenerative disease research
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