The unmatched resolution due to the high
density of data points is very beneficial for
antibody engineering projects. Multiple
transitions can be identified and determined
with high accuracy and reproducibility.
Dr. Thomas Schubert, 2bind GmbH
Look at conformational and colloidal stability simultaneously in the most precise way possible.
Test different buffer formulations with varying pH, salts, ions, or detergents that influence stability
Detect subtle unfolding transitions to pinpoint slight differences between antibody candidates
Identify the storage and formulation conditions that cause aggregation and impact stability
THERMAL UNFOLDING AGGREGATION
Conformational stability doesn't always correlate with colloidal stability. Here, the lowest pH destabilizes the mAb, yet
reduces it's propensity to aggregate. Conversely, the highest pH stabilizes the mAb, but causes an increase in the level of
aggregation. Therefore, selecting a pH between the two extremes may be the better formulation compromise to use.
Optimize antibody developability
using Prometheus, the new gold standard
for characterizing antibody stability
