5
TECHNICAL NOTE
©2019 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved.
Figure 3: Results from four
selected buffer conditions
generating both high and
low Signal Quality (SQ).
TRIC traces are plotted as
relative fluorescence over
time in seconds. Green
traces represent reference
samples (5 nM G9a), while
purple traces represent
positive controls
(5 nM G9a + 100 μM SAM).
- 1 0 1 2 3 4 5
T i m e [ s ]
0 . 9 4
0 . 9 5
0 . 9 6
0 . 9 7
0 . 9 8
0 . 9 9
1
R
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v
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F
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s
c
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n
c
e
[
-
]
- 1 0 1 2 3 4 5
T i m e [ s ]
0 . 9 4
0 . 9 5
0 . 9 6
0 . 9 7
0 . 9 8
0 . 9 9
1
R
e
l
a
t
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v
e
F
l
u
o
r
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s
c
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n
c
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[
-
]
- 1 0 1 2 3 4 5
T i m e [ s ]
0 . 9 4
0 . 9 5
0 . 9 6
0 . 9 7
0 . 9 8
0 . 9 9
1
R
e
l
a
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F
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[
-
]
Square:
C3
Square:
C9
Buffer Condition:
10 mM HEPES pH 7.4
150 mM NaCl
0.005 % Tween®20
SQ:
6.54
Buffer Condition:
10 mM HEPES pH 7.4
150 mM NaCl
10 mM CaCl2
0.005 % Tween®20
SQ:
18.63
Square:
F9
Buffer Condition:
10 mM HEPES pH 7.4
150 mM NaCl
5 mM EDTA
0.005 % Tween®20
SQ:
1.09
- 1 0 1 2 3 4 5
T i m e [ s ]
0 . 9 4
0 . 9 6
0 . 9 8
1
1 . 0 2
1 . 0 4
1 . 0 6
1 . 0 8
R
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[
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]
Square:
E9
Buffer Condition:
10 mM HEPES pH 7.4
150 mM NaCl
1 mM TCEP
0.005 % Tween®20
SQ:
<1
