Technical Notes

Validation of Tycho NT.6 precision and repeatability

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Tycho is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2019 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-TY-006-01 nanotempertech.com Intermediate precision mean SD RSD (CV) T i 87.57 °C 0.21 °C 0.24% Initial ratio 0.903 0.007 0.73% Sample brightness 1037.7 29.0 2.79% Table 2: Intermediate precision (inter-assay precision) was directly determined across all 234 capillaries run. TECHNICAL NOTE For more info, visit nanotempertech.com/tycho Repeatability mean intra-system SD mean intra-system RSD (CV) T i 0.1005 ± 0.0236 0.11 % ± 0.03 % Initial ratio 0.0007 ± 0.0002 0.08 % ± 0.02 % Sample brightness 13.4 ± 5.3 1.30 % ± 0.52 % Table 1: Repeatability (intra-assay precision) was calculated by determining the variations (SD/RSD) for each of the 13 systems, then calculating average within-system variations across all 13 systems.

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