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Going small to win big: Fragment-based screening in drug discovery

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19 19 Ross D, Gaitan M, Locascio LE. Temperature measurement in microfluidic systems using a temperature dependent fluorescent dye. Anal Chem. 2001 Sep 1; 73(17):4117-23. 20 Scott DE, Spry C, Abell C. Differential scanning fluorimetry as part of a biophysical screening cascade. In: Erlanson, DA, Jahnke, W, eds. Fragment-based drug discovery: Lessons and outlook. 1st ed. Wiley-VCH; 2016; 139-172. 21 Silvestre HL, Blundell TL, Abell C, Ciulli A. Integrated biophysical approach to fragment screening and validation for fragment-based lead discovery. Proc Natl Acad Sci USA. 2013 Aug 6; 110(32):12984-9. 22 Torres OB, Duval AJ, Sulima A, et al. A rapid solution-based method for determining the affinity of heroin hapten-induced antibodies to heroin, its metabolites, and other opioids. Anal Bioanal Chem. 2018 Jun; 410(16):3885-3903. 23 van den Bogaart G, Meyenberg K, Diederichsen U, Jahn R. Phosphatidylinositol 4,5-bisphosphate increases Ca2+ affinity of synaptotagmin-1 by 40-fold. J Biol Chem. 2012 May 11; 287(20):16447-53. 24 Vedadi M, Niesen FH, Allali-Hassani A, et al. Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc Natl Acad Sci USA. 2006 Oct 24; 103(43):15835-40.

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