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Going small to win big: Fragment-based screening in drug discovery

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17 1 Baaske P, Wienken CJ, Reineck P, Duhr S, Braun D. Optical thermophoresis for quantifying the buffer dependence of aptamer binding. Angew Chem Int Ed Engl. 2010 Mar 15; 49(12):2238-41 2 Bartoschik T, Bekic I, Hassemer T, et al. Improved quantitation of biomolecular interactions by MicroScale Thermophoresis using the RED-NHS 2nd Generation labeling kit. 2018. Application Note, NanoTemper Technologies, Inc. 3 Chapleau RR, Frey JS, Riddle DS, Ruiz ON, Mauzy CA (2015) Measuring single-domain antibody interactions with epitopes in jet fuel using microscale thermophoresis. Anal Lett. 2014 Oct 1; 48(3):526–530 4 Congreve M, Carr R, Murray C, Jhoti H. A 'rule of three' for fragment-based lead discovery? Drug Discov Today. 2003 Oct 1; 8(19):876-7. 5 Erlanson DA. Introduction to fragment-based drug discovery. Top Curr Chem. 2012; 317:1-32. 6 Gupta AJ, Duhr S, Baaske P. Microscale Thermophoresis (MST). In: Roberts G, Watts A, eds. European Biophysical Societies Encyclopedia of Biophysics. Springer, Berlin, Heidelberg; 2018; 1-5. References

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