Application Notes

Quantifying Oligonucleotides Binding to Human Serum Albumin with MST: A Faster and Precise Approach for Drug Candidates ADME Profiling

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Monolith is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2019 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. NT-MO-035 APPLICATION NOTE nanotempertech.com References 1 Varshney, Ankita, et al. "Ligand binding strategies of human serum albumin: how can the cargo be utilized?" Chirality: The Pharmacological, Biological, and Chemical Consequences of Molecular Asymmetry 22.1 (2010): 77-87. 2 Fanali, Gabriella, et al. "Human serum albumin: from bench to bedside." Molecular aspects of medicine 33.3 (2012): 209-290. 3 Fasano, Mauro, et al. "The extraordinary ligand binding properties of human serum albumin." IUBMB life 57.12 (2005): 787-796. 4 Manoharan, Muthiah, et al. "Improving antisense oligonucleotide binding to human serum albumin: Dramatic effect of ibuprofen conjugation." ChemBioChem 3.12 (2002): 1257-1260. 5 Crooke, Stanley T. "Basic principles of antisense therapeutics." Antisense research and application. Springer, Berlin, Heidelberg, 1998. 1-50. 6 Hellman, Lance M., and Michael G. Fried. "Electrophoretic mobility shi assay (EMSA) for detecting protein–nucleic acid interactions." Nature protocols 2.8 (2007): 1849. 7 Alves, Carolina, and Celso Cunha. "Electrophoretic mobility shi assay: Analyzing protein-nucleic acid interactions." Gel Electrophoresis- Advanced Techniques. InTech, 2012. 8 Garidel, Patrick, Claudia Hoffmann, and Alfred Blume. "A thermodynamic analysis of the binding interaction between polysorbate 20 and 80 with human serum albumins and immunoglobulins: A contribution to understand colloidal protein stabilisation." Biophysical chemistry 143.1-2 (2009): 70-78.

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