Application Notes

Rapid quantification of unfolded proteins for quality control and optimization of storage conditions

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APPLICATION NOTE Prometheus is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. NT-PR-004-2 nanotempertech.com References 1. Amaning K, Lowinski M, Vallee F, Steier V, Marcireau C, Ugolini A, Delorme C, Foucalt F, McCort G, Derimay N, Andouche C, Vougier S, Llopart S, Halland N, Rak A (2013) The use of virtual screening and differential scanning fluorimetry for the rapid identification of fragments active against MEK1. Bioorganic & medicinal chemistry letters 23: 3620-3626 2 Breitsprecher D, Linke P, Roth HM, Rak A (2014) Case Study on Automated Screening Project of a Fragment Library against MEK1 kinase. Application Note NT_MO-021 3 Maschberger M, Hüttl S, Müller TD, Breitsprecher D (2015) Detergent Screen for solubilized membrane proteins – Case study on the SLAC-protein HiTehA from Haemophilus influenzae. Application Note NT-PR-002 4 Oyarzabal J, Zarich N, Albarran MI, Palacios I, Urbano-Cuadrado M, Mateos G, Reymundo I, Rabal O, Salgado A, Corrionero A, Fominaya J, Pastor J, Bischoff JR (2010) Discovery of mitogen-activated protein kinase-interacting kinase 1 inhibitors by a comprehensive fragment-oriented virtual screening approach. Journal of medicinal chemistry 53: 6618-6628 5 Raynal B, Lenormand P, Baron B, Hoos S, England P (2014) Quality assessment and optimization of purified protein samples: why and how? Microbial cell factories 13: 986

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