APPLICATION NOTE
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All replicates of the positive control SAM (yellow dots) show a significant
signal amplitude and very little variability throughout the considerably large
single-dose screen, spanning sixteen 384-microwell plates. Some data points
were categorized as autofluorescence (purple dots) or aggregation (green
dots), both categories part of the quality checks performed automatically
by the so ware. Molecules interacting with the target were identified as hits
(light blue dots).
Figure 2 shows the summary of the results seen in the scatter plot. The majority
of the fragments (77.1%) were automatically identified by the so ware as
non-binders since their signals fell within the area of insignificant ΔFnorm,
Control
Hit
Aggregation
Auto-uorescence
Non-binder
Inconclusive
Area of insignicant ∆Fnorm
-100
-50
0
50
100
∆Fnorm
[‰]
Index
5000 4000 3000 2000 1000 0
Figure 1: Scatter plot showing the ΔFnorm response of all tested fragments in duplicate. The points are colored
corresponding to the category they were sorted into by the so ware algorithm.
