Use proven technology that's been
around for over 10 years
Quantifying molecular interactions — measuring how tight
or weak a ligand binds to its target — via Temperature
Related Intensity Change (TRIC) isn't new. It's done by
labeling your target molecule with a fluorescent dye and
mixing it with your ligand. Then, a very precise and brief
laser-induced temperature change is applied, causing a
variation in fluorescence intensity which is impacted if your
ligand binds to your target. This change in fluorescence is
measured and plotted against your ligand concentration to
obtain the dissociation constant or K
d
.
Decide which hits are worth
moving forward with sooner
Generating results is great, but getting automated,
actionable insights from your results is even better.
Dianthus DI.Screening Analysis so ware gives you
screening summaries as well as easy-to-interpret
ranking tables and histograms. Quickly compare
K
d
s and decide which candidates are worth moving
forward with sooner rather than later.
Non-binder
77.03%
Potential Hit
9.2%
Not Reproducible
6.83%
Inhomogeneous
Fnorm
Scan Anomaly
Auto-fluorescence
Aggregation
Hit
3.53%
Relative
Fluorescence
Time (s)
0 1 2 3 4 5
0 .8 0
0 .8 5
0 .9 0
0 .9 5
1 .0 0
1 .0 5
F
norm
(‰)
Ligand concentration (M)
1 E-⁶ 1 E-⁵ 1 E-⁴ 1 E-³
8 5 0
8 6 0
8 7 0
8 8 0
8 9 0
9 0 0
9 1 0
1 E-²
K
d
