Technical Notes

Repeatability assessment of Tycho NT.6 for better protein quality checks

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TECHNICAL NOTE Repeatability assessment of Tycho NT.6 for better protein quality checks Mariam Mohamadi, Nuška Tschammer and Dennis Breitsprecher NanoTemper Technologies GmbH, Floessergasse 4, 81369 Munich Abstract Repeatability or the precision at which a measurement is collected is a key consideration when evaluating and interpreting data. Having a strong confidence in the reliability of the results leads to better decision making and more efficient experimental workflows. The Tycho™ NT.6 system provides a quick and precise method to monitor protein quality. In minutes, researchers are able to make better educated decisions on the quality of their sample materials and in turn improve their expression, purification and characterization workflows. Introduction and Results Reproducing results can be the greatest scientific challenge that researchers face. Amazing discoveries can turn into failure if the results can not be reproduced in other scientist's hands. The first step to generating reproducible results is the ability to identify and characterize the quality of the starting material that is being used.

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