TECHNICAL NOTE
Tycho is a trademark
of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich,
Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-TY-004-01
nanotempertech.com
Average SD RSD (%)
T
i
1 74.4 °C +/- 0.07 °C +/- 0.09
T
i
2 86.2 °C +/- 0.06 °C +/- 0.07
Initial ratio 0.837 +/- 0.001 +/- 0.11
Table 1: Thermal unfolding profile analysis of trastuzumab over a time period
of 17 days. Measurement values represent means from 24 measurements.
SD=standard deviation, RSD=relative standard deviation.
Conclusions
Reproducibility is a common issue faced by all
scientists. The Tycho NT.6 system offers high
repeatability and precise measurements when
examining relative protein stability and quality. In
the example shown using the monoclonal antibody
trastuzumab and running 24 replicates, the measured
temperature inflection points (T
i
1 and T
i
2) had a
standard deviation that was less than 0.1°C. This
level of precision and repeatability is unmatched
by any current technology that examines protein
quality. Tycho NT.6 has unrivaled capability to
provide accurate and reliable protein stability results,
offering researchers an invaluable tool for higher data
reproducibility.
References
1. Freedman, L. P., et. al., The need for improved education and
training in research antibody usage and validation practices.
Biotechniques, 61: 16-18m 2016.
2. Weller, M.G., Quality issues of research antibodies., Analytical
Chemistry Insights, 11:21-27, 2016.