Technical Notes

Repeatability assessment of Tycho NT.6 for better protein quality checks

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TECHNICAL NOTE Tycho is a trademark of NanoTemper Technologies GmbH, Munich, Germany. NanoTemper is a registered trademark of NanoTemper Technologies GmbH, Munich, Germany. ©2017 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-TY-004-01 nanotempertech.com Average SD RSD (%) T i 1 74.4 °C +/- 0.07 °C +/- 0.09 T i 2 86.2 °C +/- 0.06 °C +/- 0.07 Initial ratio 0.837 +/- 0.001 +/- 0.11 Table 1: Thermal unfolding profile analysis of trastuzumab over a time period of 17 days. Measurement values represent means from 24 measurements. SD=standard deviation, RSD=relative standard deviation. Conclusions Reproducibility is a common issue faced by all scientists. The Tycho NT.6 system offers high repeatability and precise measurements when examining relative protein stability and quality. In the example shown using the monoclonal antibody trastuzumab and running 24 replicates, the measured temperature inflection points (T i 1 and T i 2) had a standard deviation that was less than 0.1°C. This level of precision and repeatability is unmatched by any current technology that examines protein quality. Tycho NT.6 has unrivaled capability to provide accurate and reliable protein stability results, offering researchers an invaluable tool for higher data reproducibility. References 1. Freedman, L. P., et. al., The need for improved education and training in research antibody usage and validation practices. Biotechniques, 61: 16-18m 2016. 2. Weller, M.G., Quality issues of research antibodies., Analytical Chemistry Insights, 11:21-27, 2016.

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