Technical Notes

Better optimization of biosensor assay development with Tycho NT.6

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TECHNICAL NOTE Better optimization of biosensor assay development with Tycho™ NT.6 Dennis Breitsprecher and Nuska Tschammer NanoTemper Technologies GmbH, Floessergasse 4, 81369 Munich Abstract Surface plasmon resonance (SPR) is an optical methodology used to detect and quantify molecular interactions. Although SPR is widely used and considered by many researchers to be the gold standard for quantification of protein interactions, assay optimization can be technically challenging, time-consuming and costly. The Tycho NT.6 system quickly analyzes different conditions typically tested when optimizing an SPR assay. It utilizes very small amounts of sample and provides dramatic time and cost savings compared to standard SPR assay development procedures. Tycho NT.6 is simple to use and enables researchers to make better educated decisions in developing and optimizing their binding interaction assays. Introduction Surface plasmon resonance (SPR) and other biosensor-based analytical methods are standard tools used in academic and pharmaceutical research laboratories for the quantification of protein interactions. Typically, one binding partner – the ligand – is covalently immobilized on the surface of a biosensor chip, while the other binding partner – the analyte – is added in solution to measure binding kinetics. Covalent immobilization of proteins to the biosensor surface is o en problematic, since acidic, salt-free buffers are required for optimal coupling using lysine chemistry. These harsh conditions can negatively influence protein integrity and function.

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