9
Surface Plasmon
Resonance
(SPR)
Isothermal
Titration
Calorimetry
(ITC)
BioLayer
Interferometry
(BLI)
MicroScale
Thermophoresis
(MST)
Requires high amount of sample No Yes No No
Label-free Yes Yes Yes Optional
Immobilization-free No Yes No Yes
High throughput Yes No Yes Yes
Easy to use No No Yes Yes
Instrument costs High Low Medium Medium
Consumable costs High Low Medium Medium
Measures binding kinetics Yes No Yes No
If you've determined that you need an instrument that can measure binding affinity, there are some simple steps
you can take to find out which one would be right for your lab. Sometimes, just the thought of purchasing new
technology can be overwhelming—it can be a costly and significant decision for your lab. But you can make the
process much easier for yourself by taking the time to think about your needs and how the instrument could fit into
your established workflow. What are your research or project goals? What is most important to you? What are your
Technologies for
measuring binding affinity
How do I evaluate
my research needs?
